ARS Tris-EDTA, 90ml Vial

ARS Tris-EDTA solution for treating bacterial endometritis in mares.  The 90 ml vial with sealed rubber stopper provides three treatments when following the protocols provided in the "Formulary and Protocols in Equine Reproduction" book published by the Colorado State University Equine Reproduction Laboratory. 

Bacterial endometritis is an important cause of reduced fertility in mares.

Addition of Tris-EDTA to an antibiotic has been shown to significantly reduce the minimal inhibitory concentration (MIC) of the antibiotic in the prevention of bacterial growth in vitro.

Infusion of Tris-EDTA into the uterus of mares with bacterial endometritis followed by uterine lavage the next day has also been advocated for biofilm disruption prior to infusion of antibiotics.

This product should be used under the advise and recommendation of a veterinarian.

Automatic discounts apply when buying 5-9 (5%) and 10+ (10%) bottles.

ARS Tris-EDTA and Intrauterine Antibiotic Therapy

• Bacterial endometritis is an important cause of reduced fertility in mares.

• Equine uterine pathogens live in both a free-floating and biofilm state. Biofilms protect pathogens from the host immune system.

• Tris-EDTA has been shown to potentiate the efficacy of antibiotics in the killing of bacteria and the disruption of bacterial biofilms.

• Tris-EDTA works in part by chelating Mg²⁺ and Ca²⁺ ions from the outer cell wall of Gram-negative bacteria, allowing for greater penetration of antibiotics and consequently enhanced bacterial killing.

• Addition of Tris-EDTA to an antibiotic has been shown to significantly reduce the minimal inhibitory concentration (MIC) of the antibiotic in the prevention of bacterial growth in vitro.

• Infusion of Tris-EDTA into the uterus of mares with bacterial endometritis followed by uterine lavage the next day has also been advocated for biofilm disruption prior to infusion of antibiotics.

• This product should be used under the advise and recommendation of a veterinarian.

Tris-EDTA Protocol for Equine Intrauterine Antibiotic Infusion

*Addition of Sodium Bicarbonate is needed to buffer the pH of aminoglycoside antibiotics.

References:

Ferris RA, Wittstock SM, McCue PM, Borlee BR. Evaluation of biofilms in Gram-negative bacteria isolated from the equine uterus. J Equine Vet Sci 2014;34:121.

Ferris RA, McCue PM, Borlee GI, Loncar KD, Hennet ML, Borlee BR. In vitro efficacy of nonantibiotic treatments on biofilm disruption of Gram-negative pathogens and an in vivo model of infectious endometritis utilizing isolates from the equine uterus. Journal of Clinical Microbiology 2016;54(3):631-9.

Loncar KD, Ferris RR, McCue P, Borlee BR, Hennet ML, Borlee GI. In vitro efficacy of non-antibiotic agents against bacterial biofilm from equine uterine isolates. Proceedings of the American Association of Equine Practitioners 2015;61:458-459.